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Image Search Results


Journal: Cell reports

Article Title: Actinin BioID reveals sarcomere crosstalk with oxidative metabolism through interactions with IGF2BP2

doi: 10.1016/j.celrep.2021.109512

Figure Lengend Snippet:

Article Snippet: For overexpression experiments, PCR amplified products of actinin (primer sequences in ) were cloned into a modified pLenti CMV backbone (Addgene 17448) using HIFI DNA Assembly Master Mix (NEB 26261S) and propagated in NEB Stable E.coli (NEB C3040) ( ).

Techniques: SYBR Green Assay, In Situ Hybridization, Reporter Assay, Software, Recombinant, Protease Inhibitor